Glomerular deposition of C4d in focal segmental glomerulosclerosis and IgM-nephropathy (results of C4d staining of kidney biopsies in native kidney diseases)
Abstract
Background. C4d staining of peritubular capillaries is useful marker of antibody-mediated rejection in kidney transplant biopsies. However the usefulness of C4d-findings in native kidney biopsies have not been extensively studied. Methods. 81 biopsies from 80 patients with different pathology were stained by indirect IF method, using murine monoclonal anti-human C4d Ab (Quidel, Metro Biosystems). Results. Granular pattern of C4d deposition in glomeruli was detected in 4 of 4 lupus cases (2-class IV and 2-class V) and in 3 of 3 cases of idiopathic membranous nephropathy on periphery of capillary loops, possibly corresponding to deposits location. Linear staining of capillary loops and mesangium in all glomeruli was detected in 13 from 17 biopsies of FSGS cases, in 8 from 9 cases of IgM-nephropathy. Biopsies with minimal change disease were negative in 5 from 7 cases (two positive cases have only focal mild deposition of C4d). Most other cases show no significant C4d deposition in glomeruli - IgA-nephropathy (5 cases), myeloma kidney (2), amyloidosis (1), AIN (3), ATN (3), toxic nephropathies (3), diabetic nephropathy (2), pauciimmune crescentic GN (3), TBMD (3). Conclusion. C4d deposition in glomeruli in MGN was described earlier, but C4d deposition in FSGS cases is intriguing findings, which need further investigation. But preliminary results suggest that C4d glomerular staining could be useful marker for differential diagnosis between FSGS and MCD.
About the Authors
A. V. Sukhanov
НИИ трансплантологии и искусственных органов МЗ РФ, г. Москва
Russian Federation
Russian Federation
P. . Campbell
Университет провинции Альберта, Эдмонтон, Канада
Russian Federation
Russian Federation
Solez Kim
Университет провинции Альберта, Эдмонтон, Канада
Russian Federation
Russian Federation
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Review
For citations:
Sukhanov A.V., Campbell P., Kim S. Glomerular deposition of C4d in focal segmental glomerulosclerosis and IgM-nephropathy (results of C4d staining of kidney biopsies in native kidney diseases). Nephrology and Dialysis. 2003;5(1):42-45. (In Russ.)
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