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Том 5 №3 2003 год - Нефрология и диализ
Novel role for renal Na, K-ATPase as gene-regulator
Oleg Aizman
Аннотация: Recent studies have indicated that Na, K-ATPase may, in addition to being the key regulator of intracellular Na+ and K+-concentration, act as a signal transducer. The biological role for ouabain, the natural ligand of Na, K-ATPase, has, despite extensive research, not been well understood. We have reported that exposure of rat proximal tubular cells (RPTC), to doses of ouabain that inhibit the Na, K-ATPase activity less than 50% (10 nM - 500 µM), will induce intracellular [Ca2+]i oscillations and that this calcium signal leads to activation of the transcription factors NF-κβ. The ouabain induced calcium oscillations were blocked by an inhibitor of the IP3 receptors but not by phophospholipase С inhibitors nor by cellular depletion of IP3, suggesting that the calcium signal is not due to phospholipase С mediated IP3 release. Fluorescence resonance energy transfer (FRET) studies suggested a close proximity between the Na, K-ATPase and IP3 receptor. Our findings demonstrate a novel principle for calcium signaling via Na, K-ATPase.
Для цитирования: Oleg Aizman Novel role for renal Na, K-ATPase as gene-regulator. Нефрология и диализ. 2003. 5(3):221a. doi:
