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Subpopulations of T- and B-lymphocytes in patients with end-stage chronic kidney diseases

https://doi.org/10.28996/2618-9801-2018-4-385-393

Abstract

Patients with end-stage chronic kidney diseases are prone to infectious complications due to suppression of the immune system. The aim of the study was to identify changes in subpopulations of T- and B-cells depending on the duration of renal replacement therapy in patients with chronic kidney disease stage 5 (CKD-5). Materials and methods: twenty-one hemodialysis patients with CKD-5 and without infectious complications were studied (median age 43, m/f ratio was 15/6). The patients were divided into 3 groups depending on the duration of the hemodialysis. The 1st group included patients who were on hemodialysis from 1 to 2 years (n=6); in the 2nd group - from 2 to 3 years (n=8); in the 3rd - 3 and more years (n=7). The 20 healthy blood donors were used as controls. The percentage and absolute numbers of T- and B-lymphocytes' subsets were determined in patients' pre-dialysis peripheral blood samples and in blood samples of healthy donors by flow cytometric analysis (BD FACSCanto II). Results: the changes in subsets of T- and B-cells were not detected in patients who received hemodialysis up to 3 years. In patients with long-term hemodialysis (3 and more years) the number of CD3+, CD4+, CD8+ lymphocytes, NK-cells, B-cells subsets and the ratio of CD4/CD8 did not differ from the control group. But the absolute number of Naive CD4+, and the percentage of Effector memory CD4+ cells, and the total number of Treg cells and of Naive CD8+ cells were decreased. The percentage number of the Сentral memory CD4+ and CD8+ cells and the total number of the Terminal effector CD4+ cells were increased. Conclusion: the data obtained allow us to better understand the pathogenesis of cellular immunity changes in patients with CKD-5. This could help to correct such changes and therapeutic tactics in the future

About the Authors

B. T. Dzhumabaeva
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


L. S. Biryukova
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


N. M. Kapranov
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


I. V. Galtseva
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


Yu. O. Davydova
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


N. B. Purlo
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


L. R. Karapetyan
National medical research center of Hematology, Moscow, Russian Federation
Russian Federation


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Review

For citations:


Dzhumabaeva B.T., Biryukova L.S., Kapranov N.M., Galtseva I.V., Davydova Yu.O., Purlo N.B., Karapetyan L.R. Subpopulations of T- and B-lymphocytes in patients with end-stage chronic kidney diseases. Nephrology and Dialysis. 2018;20(4):385-393. (In Russ.) https://doi.org/10.28996/2618-9801-2018-4-385-393

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ISSN 1680-4422 (Print)
ISSN 2618-9801 (Online)