High-frequency ultrasound Doppler system for study of renal blood flow during ischemia/reperfusion of kidney
Abstract
Ischemic injury of the kidney is the leading cause of acute kidney failure (AKF). Using high frequency Doppler technique we explored the central and renal hemodynamics after 40-min ischemia followed by reperfusion (I/R) of the rat kidney. One minute after I/R an essential drop of a total renal blood flow (70% of pre-ischemic values), lowering of the terminal diastolic blood flow rate (67% of pre-ischemic values) and rise of the renal artery resistance index (0,76 before I/R and 0,92 after I/R) were observed. These changes were retained for at least 30 min. At the same time, the changes of the blood flow indexes in abdominal aorta were not significant. Ischemic preconditioning of the kidney (4 cycles of 5-min ischemia followed by 5-min reperfusion each) totally prevented the changes of hemodynamics caused by 40-min I/R. The study of venous output showed very high sensitivity of a renal blood flow to short-term episodes of ischemia.
About the Authors
S. S. Jankauskas
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University; Research Institute of Mitoengineering, Lomonosov Moscow State University
Russian Federation
Russian Federation
D. D. Matsievsky
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
E. Y. Plotnikov
Research Institute of Mitoengineering, Lomonosov Moscow State University; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
D. B. Zorov
Research Institute of Mitoengineering, Lomonosov Moscow State University; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
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Review
For citations:
Jankauskas S.S., Matsievsky D.D., Plotnikov E.Y., Zorov D.B. High-frequency ultrasound Doppler system for study of renal blood flow during ischemia/reperfusion of kidney. Nephrology and Dialysis. 2014;16(1):169-175. (In Russ.)
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